Prenatal genetic counselling for psychiatric disorders

Psychiatric disorders like schizophrenia, bipolar disorder, depression, anxiety, and obsessive–compulsive disorder are common disorders with complex aetiology. They can exact a heavy toll on the individual with the condition and can have significant impact on family members too. Accordingly, psychiatric disorders can arise as a concern in the prenatal context – couples may be interested in learning about the chance for their child to develop the illness that manifests in the family and may be interested in discussing options for prenatal testing. However, the complex nature of these conditions can present challenges for clinicians who seek to help families with these issues. We established the world's first specialist genetic counselling service of its kind in Vancouver, Canada, in 2012, and to date, have provided counselling for ~500 families and have demonstrated increases in patients' empowerment and self efficacy after genetic counselling. We draw on our accumulated clinical experience to outline the process by which we approach prenatal genetic counselling for psychiatric disorders to assist other clinicians in providing thoughtful, comprehensive support to couples seeking out this service. © 2016 John Wiley & Sons, Ltd.     

Comparative diet selection by cattle and sheep grazing two contrasting heathland communities

In the UK the funding mechanism for moorland restoration is being implemented primarily through agri-environment schemes, yet to date remarkably few comparative grazing studies with domesticated livestock have been conducted on this type of vegetation community. In this experiment the diet composition of four groups of animals grazing heathland swards with low (8%) and high (61%) percentages of cover of Calluna vulgaris was estimated from faeces profiles of n-alkanes and long-chain fatty alcohols. Two breeds of sheep (Welsh Mountain (WM) and Scottish Blackface (SBF)), and two breeds of cattle (Welsh Black (WB) and Continental cross (CX)) grazed the experimental sites during two separate 14-day sampling sessions, in July and September respectively. Both species were selective feeders, consuming grasses (selectivity indices 0.20–1.0) in preference to dwarf shrubs (selectivity indices 0.20–1.0), but there were significant differences in the proportions of various plant groups within the diets. Although the sheep diets contained significantly more C. vulgaris, the quantities consumed were small (<10% of the diet), even on the High site, reflecting the biomass of the graminoid species present, and hence availability of preferred items. The SBF sheep were the only animal type to increase consumption of C. vulgaris later in the season. There were a number of significant differences in the composition of the diets selected by the two hill breeds of sheep used in the study, with the SBF sheep recorded as consuming more C. vulgaris. These results imply that through choice of breed land managers may be able to meet environmental goals more effectively without compromising returns from conventional production systems. In contrast, the overall diet composition of the two breed types of cattle was similar. This indicates that commercial breeds have the potential to deliver the types of environmental benefits associated with grazing of traditional breeds.     

Effects of residual antibiotics in groundwater on Salmonella typhimurium: changes in antibiotic resistance, in vivo and in vitro pathogenicity

An outbreak-causing strain of Salmonella enterica serovar Typhimurium was exposed to groundwater with residual antibiotics for up to four weeks. Representative concentrations (0.05, 1, and 100 μg L(-1)) of amoxicillin, tetracycline, and a mixture of several other antibiotics (1 μg L(-1) each) were spiked into artificially prepared groundwater (AGW). Antibiotic susceptibility analysis and the virulence response of stressed Salmonella were determined on a weekly basis by using human epithelial cells (HEp2) and soil nematodes (C. elegans). Results have shown that Salmonella typhimurium remains viable for long periods of exposure to antibiotic-supplemented groundwater; however, they failed to cultivate as an indication of a viable but nonculturable state. Prolonged antibiotics exposure did not induce any changes in the antibiotic susceptibility profile of the S. typhimurium strain used in this study. S. typhimurium exposed to 0.05 and 1 μg L(-1) amoxicillin, and 1 μg L(-1) tetracycline showed hyper-virulent profiles in both in vitro and in vivo virulence assays with the HEp2 cells and C. elegans respectively, most evident following 2nd and 3rd weeks of exposure.     

Production of a refined biooil derived by fast pyrolysis of chicken manure with chemical and physical characteristics close to those of fossil fuels

The chemical and physical properties of raw biooils prevent their direct use in combustion engines. We processed raw pyrolytic biooil derived from chicken manure to yield a colorless refined biooil with diesel qualities. Chemical characterization of the refined biooil involved elemental and several spectroscopic analyses. The physical measurements employed were viscosity, density and heat of combustion. The elemental composition (% wt/wt) of the refined biooil was 82.7 % C, 15.3 % H, 0.2 % N and 1.8 % O, no S. Its viscosity was 0.006 Pa.s and a heat of combustion of 43 MJ kg^?1. The refined biooil fraction contains n-alkanes, ranging from n-C₁₄ to n-C₂₇, alkenes varying from C_10:1 to C_22:1, and long-chain alcohols. The refined biooil makes a good diesel fuel due to its chemical and physical properties.     

Separation and identification of heterocyclic nitrogen compounds in biooil derived by fast pyrolysis of chicken manure

N-heterocyclics were separated from a biooil, generated by the pyrolysis of chicken manures by column chromatography over neutral alumina and silica, and identified by Pyrolysis Field Ionization Mass Spectrometry (Py-FIMS) and Electrospray Ionization Mass Spectrometry (ESI-MS). Identities of chemical structures, whose presence was indicated by ESI-MS, were confirmed by comparing the Collision-Induced Dissociations (CID's) mass spectra of unknown and standards. The following seven base structures were identified: pyrazine, benzoquinoline, carbazole, phenylpyridine, indole, pyrazole and pyridine. Available hydrogens bonded to ring carbons and nitrogens on the seven N-heterocyclics were increasingly substituted by alkyl groups, mainly methylene groups (m/z 14) to yield mono-, di-, tri- methyl N-heterocyclics. In some instances, longer alkyl chains, such as ethyl, propyl, up to heptyl groups were the substituents.     

Review and meta-analysis of 82 studies on end-of-life management methods for source separated organics

This article reports on a literature review and meta-analysis of 82 studies, mostly life cycle assessments (LCAs), which quantified end-of-life (EOL) management options for organic waste. These studies were reviewed to determine the environmental preferability, or lack thereof, for a number of EOL management methods such as aerobic composting (AC), anaerobic digestion (AD), gasification, combustion, incineration with energy recovery (often denoted as waste-to-energy incineration), mechanical biological treatment, incineration without energy recovery (sometimes referenced by just the word “incineration”), and landfill disposal with and without energy recovery from generated methane. Given the vast differences in boundaries as well as uncertainty and variability in results, the LCAs among the 82 studies provided enough data and results to make conclusions regarding just four EOL management methods – aerobic composting, anaerobic digestion, mass burn waste-to-energy (WTE), and landfill gas-to-energy (LFGTE). For these four, the LCAs proved sufficient to determine that aerobic composting and anaerobic digestion are both environmentally preferable to either WTE or LFGTE in terms of climate change impacts.For climate change, LCA results were mixed for WTE versus LFGTE. Furthermore, there is a lack of empirically reliable estimates of the amount of organics input to AD that is converted to energy output versus remaining in the digestate. This digestate can be processed through aerobic composting into a compost product similar to the compost output from aerobic composting, assuming that the same type of organic materials are managed under AD as are managed via AC. The magnitude of any trade-off between generation of energy and production of compost in an AD system appears to be critical for ranking AC and AD for differing types of organics diversion streams. These results emphasize how little we generally know, and exemplify the fact that in the reviewed literature no single EOL management method consistently topped all other management options across all environmental impacts, and that future studies must strive to match existing analytical boundaries and alternatives assessed to increase knowledge if as a community we expect to be able to make even more generalized conclusions.     

A framework for assessing the impact of the IPPC directive on the performance of the pig industry.

New European Union legislation on Integrated Pollution Prevention and Control (IPPC) is being implemented in the United Kingdom, enacted by the Pollution Prevention and Control Act 1999 and its statutory instruments, the Pollution Prevention and Control (England and Wales) Regulations 2000. This legislation incorporates previously unregulated installations in the food and intensive livestock sectors, such as pig installations above a given threshold size. IPPC requires that installations adopt Best Available Techniques and follow General Binding Rules of good practice in order to manage their environmental effects. IPPC has significant potential impacts for both the environmental and financial performance of the pig industry. In this context, the paper discusses the IPPC implementation process as it applies to the sector and presents a methodological framework for assessing the environmental and cost benefit implications of the new regulations.     

A fully reproductive fighting morph in a soldier clade of gall-inducing thrips (Oncothrips morrisi)

We present the first life history data for Oncothrips morrisi, a species in a clade of haplodiploid, Australian gall-inducing thrips that has a micropteran fighting morph in the first generation of the gall. Micropterans in other species in the clade have lower fecundity than their mother, and these species are considered eusocial. There is no such reproductive skew in O. morrisi, and the species is not eusocial by any definition. The volume of O. morrisi galls is between 4 and 20 times greater in than eusocial species in the clade and the dispersing-brood size varies between 5 and 15 times larger than that of other species. The lack of skew in O. morrisi could be associated with large gall volume, reducing competition between females for space and feeding sites for their offspring. As O. morrisi is in a lineage basal to eusocial Oncothrips, we also discuss selective factors for the evolution of a non-dispersing fighting morph in this clade. The costs involved in dispersal and latency to reproduction may have lowered the selective threshold for the evolution of a non-dispersing morph. Once non-dispersal evolved, selection to defend one's offspring may have promoted the evolution of soldier-like morphology and behaviour.     

Emissions and risks associated with oxyfuel combustion: State of the science and critical data gaps

Oxyfuel combustion is a promising technology that may greatly facilitate carbon capture and sequestration by increasing the relative CO₂ content of the combustion emission stream. However, the potential effect of enhanced oxygen combustion conditions on emissions of criteria and hazardous air pollutants (e.g., acid gases, particulates, metals and organics) is not well studied. It is possible that combustion under oxyfuel conditions could produce emissions posing different risks than those currently being managed by the power industry (e.g., by changing the valence state of metals). The data available for addressing these concerns are quite limited and are typically derived from laboratory-scale or pilot-scale tests. A review of the available data does suggest that oxyfuel combustion may decrease the air emissions of some pollutants (e.g., SO₂, NOx, particulates) whereas data for other pollutants are too limited to draw any conclusions. The oxy-combustion systems that have been proposed to date do not have a conventional “stack” and combustion flue gas is treated in such a way that solid or liquid waste streams are the major outputs. Use of this technology will therefore shift emissions from air to solid or liquid waste streams, but the risk management implications of this potential change have yet to be assessed. Truly useful studies of the potential effects of oxyfuel combustion on power plant emissions will require construction of integrated systems containing a combustion system coupled to a CO₂ processing unit. Sampling and analysis to assess potential emission effects should be an essential part of integrated system tests.

Implications: Oxyfuel combustion may facilitate carbon capture and sequestration by increasing the relative CO₂ content of the combustion emission stream. However, the potential effect of enhanced oxygen combustion conditions on emissions of criteria and hazardous air pollutants has not been well studied. Combustion under oxyfuel conditions could produce emissions posing different risks than those currently being managed by the power industry. Therefore, before moving further with oxyfuel combustion as a new technology, it is appropriate to summarize the current understanding of potential emissions risk and to identify data gaps as priorities for future research.